Entomopathogenic and parasitic nematodes are lethal parasites of insects, used as biocontrol agents and have occupied a wide range of habitats. Isolation and identification of native nematodes are necessary for successful control of endemic insect pests in a particular location. The aim of this study was to reveal native nematodes from Eastern Georgia and their potential, which were found from soil and insects and for used them against pest insects. For this study, we chose two parasitic and one entomopathogenic nematode. Amphimermisthesamica sp. n. (Nematoda: Mermithidae) was isolated from the soil of gardening plot, located in village Tezami of Mtskheta-Mtianeti Region. Among many species of nematodes, we found out specimens of nematodes of the genus Labronemella Andrassy, 1985, that was described as a new species Labronemellageorgiensis sp. nov. From the restored ecosystems of Tbilisi environs. Entomopathogenic nematode - Steinernemaborjomiense n. sp. was isolated in the territory of Borjom-Kharagauli Reserve, from the host-insect cadaver. Morphological, anatomic and morphometric analysis of nematodes, isolated from different location has shown that the described nematodes are a new species. All above-mentioned nematodes were found from Eastern Georgia.
Chyrsanthemum indicum is also known as ‘bunga kekwa’ in Malay and ‘ye ju hua’ in Chinese. In Chinese traditional medicine, C. indicum has been used as anti-inflammatory, analgesic, antipyretic, and for the treatment of eye diseases. The present study was aimed to scientifically evaluate the antipyretic activity of C. indicum flowers. Water and ethanol extracts of C. indicum flowers were prepared using cold maceration method. The extracts were subjected to qualitative preliminary phytochemical analysis to identify the presence of secondary metabolites. The results of ethanol extracts showed presence of alkaloids, carbohydrates, glycosides, oils, fats, terpenoids, flavonoids, and volatile oils whereas constituents in the water extract also found to be the same as ethanol extract except the oils and fats. The antipyretic activity was evaluated by yeast-induced pyrexia model in wistar albino rats. Water and ethanol extracts of C. indicum flower were administered orally to the pyrexia-induced rats at a dose of 200 mg/kg body weight. The antipyretic activity was assessed by measuring the rectal temperature of rats using digital thermometer. Temperatures were recorded before and after the induction of yeast and after oral administration of extracts and standard drug (paracetamol 150 mg/kg) at 1h, 2h, 3h, 4h, 5h and 6h. The results showed that both water and ethanol flower extracts of C. indicum exhibits highly significant (p<0.01) antipyretic effect by decreasing the elevated rectal temperature in yeast-induced pyrexia at a dose of 200 mg/kg. These results proclaimed support scientifically the antipyretic effect of C. indicum flowers.
Anandarajagopal Kalusalingam*, Nurul Amanina Binti Ali Akbar, Abdullah Khan, Poonguzhali Subramaniam, Anbu Jeba Sunilson.