Mosquito control programs are now threatened by the selection of mosquito populations resistant to the chemical insecticides. Thus, alternative vector control methods are necessary. This study was aimed at investigating on the larvicidal activities of Carica papaya Linn latex gelon larvae of Culex quinquefasciatus in lymphatic filariasis vector control in Lokossa district in south-western Republic of Benin, West Africa. Larvae of Culex quinquefasciatus mosquitoes were collected from breeding sites using the dipping method from September to November 2024 during the small rainy season in Lokossa district. A batch of 25 larvae of fourth instar were exposed to a mixture of Carica papaya Linn latex gel containing in each of five glass jars or test cups of same dimensions with different concentrations of 1mg/l, 2mg/l, 3mg/l, 4mg/l and 5mg/l and one control jar for each serial concentrations containing no trace of Carica papaya Linn latex gel. Larval mortality was recorded after 24 hours, 48 hours and 72hours exposure. The results showed that Carica papaya Linn latex gelhad acted by poisoning the larvae of four instars which could not breathe and pupate. The use of Carica papaya Linn latex gel disallows mosquito larvae to acquire tolerance.

Mosquito control programs are now threatened by the selection of mosquito populations resistant to the chemical insecticides. Thus, alternative vector control methods are necessary. The current study was aimed to study theeffects of synergist S.S.S-tributyl-phosphorotrithioate (DEF) on fenitrothiontolerance in Anopheles gambiae sensu latolarvae from Djakotomey district in Couffo department in south-western Benin, West Africa.Larvae and pupae were collected from April to July 2024 during the great rainy season inDjakotomey district.Larval bioassays were performed on these collected Anopheles gambiaeS.L. larvae usingfenitrothion as larvicide and S.S.S-tributyl-phosphorotrithioate (DEF) as enzyme inhibitor or synergist. The results showed that esterase enzymes played a role in Anopheles gambiae S.L. larvae tolerance to fenitrothion in Djakotomey district. Esterase enzyme played a role in Anopheles gambiae sensu lato larvae tolerance to organophosphate in Couffo department.